Density Gradient Enrichment of Escherichia coli Conditional msbA Mutants
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چکیده
منابع مشابه
Density gradient enrichment of Escherichia coli conditional msbA mutants.
Insight into the mechanism of lipid transport to the outer membrane of gram-negative bacteria has been hampered by the lack of an effective genetic screen for defective mutants. This work demonstrates an enrichment of conditional mutants defective in lipopolysaccharide export by Ludox density gradient centrifugation and selection for detergent resistance. New temperature-sensitive mutants with ...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2007
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.01992-07